Molecular Study to Detect Escherichia coli in Diarrheic Children and its Antibiotic Resistance

Diarrheal diseases can lead to infections and cause morbidity mortality in children. Diarrheagenic Escherichia coli (DEC) is an etiological agent, which considered the major causative agent of diarrhea children some developing countries. The aims this work were estimate (E. coli) causing less than 5 years old, detect biofilm virulence factors effect antibiotics. For methodology, a total 112 specimens collected from two health centers, Al-Zahraa Teaching Hospital Public Health Laboratory (located Al-Kut city/ Wasit province Iraq). All grown on simple rich media. A 43 (38.4%) E. isolates identified using different traditional methods, such as biochemical tests 16S rRNA sequencing. Polymerase chain reaction (PCR) testing was used factor genes that play important role pathogenesis diarrheic e.g., rRNA, bfpA, eaeA. In study, several antibiotics sensitivity resistivity isolates. fully coli. These samples genes, 31 (72.1%) 29 (29.4%) carried bfpA eaeA, respectively. preponderance completely resistant penicillin (100%). Additionally, 33 (76.7%) 27 (62.8%) cephalothin amoxycillin-clavulanic acid, Furthermore, showed levels antibiotics, including polymyxin B 40 (93%), norfloxacin 38 (88.4%), gentamycin 26 (60.4%), meropenem 22 (51.2%). conclusion, diarrheagenic prevalent among Most varying results for presence factors. addition, all sensitive B.